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KMID : 0358420090520070724
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Korean Journal of Obstetrics and Gynecology
2009 Volume.52 No. 7 p.724 ~ p.733
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Ovarian cancer related gene targeting with large circular antisense library
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Doh Kyung-Oh
Sim Jae-Chul
Yang Hoe-Saeng
Chun Geun-Soo
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Abstract
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Objective: The aim of this study is to find out the genes which are related to ovarian cancer cell growth using large circular antisense library.
Methods: Clones for antisense library were uni-directionally sub-cloned into pBS SK (-) vector. LC-antisense molecules were then purified from the culture supernatants of the bacterial competent cells superinfected with M13K07 helper bacteriophages. The LC-antisense library to 240 unigene clone was constructed and utilized in the identification of genes functionally involved in the growth of ovarian cancer cells.
Results: The 17 numbers out of the 240 numbers of the antisense library exerted a marked inhibitory effect on the growth of SK-OV 3.
Conclusion: The putative functional categorization of each gene was then conducted via public databases. These candidates may be used as target genes for drug development or adjuvant of conventional chemotherapeutic drugs.
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KEYWORD
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Ovarian neoplasms, Large circular antisense DNA, Gene therapy
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